1.
Stalker (Half-Life 2, PC)
2.
3. A Flood (Halo, X-box and PC)
4. John Mullins (Soldier of Fortune, PC)
5. Earthworm Jim (Earthworm Jim series, various 16-bit)
6. Princess Peach (Mario 64, N64)
7. Ecco (Ecco the Dolphin, various 16-bit) Various?
8. Vectorman (Vectorman, Genesis)
9. Solid Snake (Metal Gear, MSX)
10.
GLaDOS (Portal, PC)
11. Motaro (Mortal Kombat 3, Arcade/ PSone)
12. Tails (Sonic the Hedgehog, Genesis)
13. Luke Skywalker (Lego Star Wars, The Original Trilogy, Multiplatform GC, X-box, etc.)
14. Michael Jackson (Michael Jacksons Moonwalker, Sega)
15. Donatello (TMNT, NES)
16. Jason's Mask (Friday the 13th, NES)
17. Ken (Street Fighter, Arcade/NES)
18. Pac-Man (Pac-Man, arcade)
19. Manny (Grim Fandango, PC)
20.
L5 Riesig (Battlefield 2142, PC)
21.
PIPboy (Fallout)
22. Crash Bandicoot (Crash Bandicoot, PS)
23. ET (ET: The Extraterrestial, Atari 2600)
24. a worm (Worms, PC)
25. Mr. Frosty (Clayfighter, SNES)
That in bold are my updates